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Sha Liao is the Senior Genomics Market Segment Leader at Hamilton, so with experience in medicine and biochemistry, Sha develops innovative automated solutions for the life science industry.
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Dr Liao collaborates with researchers and clinicians to streamline sample prep to answer, process and optimise liquid handling, enhancing productivity and data quality to accelerate genomic discoveries and healthcare advancements.
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So there are two, I guess, icebreaker questions.
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What are the manual challenges in your lab or in the lab in general?
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And what are automated solution workflow do you want to automate?
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So the stage is yours, looking forward to your talk.
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Good afternoon, everyone.
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Thank you for your time to attending these sessions.
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So we, you already know my background.
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So we just keep going briefly.
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We'll quickly look at the agenda.
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Today I will briefly introduce Hamilton company.
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If you don't know who we are, we are other than the challenge and the Hamilton solution for the genomic research and then we move on the challenge and the Hamilton solution for the single cell analysis, omics and the spatial biology.
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Then we bravely touch the solution for the proteomics.
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Hampton is a global leader in the liquid handler and live automation.
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We have a rich history spanned over 75 years, over 3000 dedicated team member across the global.
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We have 22 offices and the Hamilton begin with Mr.
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Clark Hamilton who developed the first Microliter Syringe.
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Our company established in US in 1955 and was founded in Switzerland in 1966.
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Hamilton core principle is innovation, quality, service and the support all those value drive everything we do daily and we offer the unique and the comprehensive product portfolio.
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We can have the small footprint life complex platform up to very advanced interrogatable platform.
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So you can see here we have the micro lab (ML) Prep, this has 8 SBI space and up to ML Vantage this has 72 SBI space deck.
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And then down the logical cabinet you can integrate.
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And also this advantage you can do back-to-back, side to side integration.
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So we can offer modular up to scalable very advanced solution.
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We offer the solution.
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We don't want to limit what you do.
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So we really take the customer need very seriously and then we all know the challenge in genomic.
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I'm sure you all kind of expert in the genomic workflow.
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I just want to quickly go through what are the solution we offer.
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So for the you know genomic you have sample all different kind of sample, then you may do the sample prep.
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Sample prep may include a deperophene capping, the capping when you deal with the saliva tubes and also I apologise, I think maybe this colour is harder to read.
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And then we have the easy blood for the bladder fractionation and then you will do the nucleic acid extraction.
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Then the extraction the sample quality is really impact your downstream.
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I say for the downstream, you can do the PCR or digital PCR, anything related to the and the point the PCR then do the library prep with the enrichment.
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Then you can go the short read along read with the Idumina pack bio nanopor.
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And also we offer the solution for the bionano for the optical genomic mapping, we have the solution.
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So you can do the labelling and standing on the back we have automated solution too.
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So we all know the challenger for the nucleic acid extraction or different sample tab and the sample quality and that the throughput need and the development timing for the long reader you want to sharing your DNA to the right size.
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And then the troubleshooting cost for the nucleic acid extraction, maybe you know the development or troubleshooting cost is not a significant, but move to the NGS could be really significantly costly.
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So to handle all those different needs, Hamilton has all different solution for each single step or in the genomic workflow.
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First, I just briefly talk about the sample prep.
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So we have the system what we call the blood fractionation.
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All you here is easy blood.
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We offer the two method to do the blood fractionation is 1 is the image based and the second is a liquid of recognition.
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We have the pressure sensor on each our single pipette channel.
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So with a different face, you will generate a different pressure due to the liquid, liquid interface by the density differential.
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And then we collaborate with the Covaris, we create a code sonication star that is a workflow will be active deparaffin and also do the RNA DNA extraction with the Covaris Sonicator on the right-hand side and the Hamilton Decapper on the left-hand side.
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So the whole workflow is just a worker away.
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So basically sonicate then you will put the plate on the deck, do the pipetting and the metrics tube will capping the capping use of the capper.
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So we collaborate with the call LabCorp to create a solution and the latest today's system first kind of funny story, they come back to us and say they have a LabCorp have existing method, right.
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So they use this system, they come back to us and say your system is too good and the yield is a shoot off the roof so now we have a new problem.
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We have to dilute it.
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So then they found out used to be they have they use FFPE, they do 24, they scratch 22 times.
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But right now with the Sonication STAR, they only need to do 6 score.
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They can generate the yield match to their pure is the method.
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So really save the time and the resource here is whatwe call the live elite decapper.
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These can scan the barcode for the sample traceability when you already integrate with the liquid handler.
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So for the nucleotide, I see the solution we have a few.
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This one we call the MagEx STARline.
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STARline is up to 96 sample process and the star can do 496 plates.
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It's a magnetic copy based.
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We open to any, you know, magnetic be based solution.
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So we now to lock down the chemistry.
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As I say, we want to meet your need.
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We provide a solution.
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You want it.
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We don't want to limit it.
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You and then also Hamilton collaborate with some of Fisher, some of Fisher integrated with some of Thermo Fisher Presto.
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The reason we have those two different magnetic bead based extraction is this one is you know for the supernatated remove or then the thermal solution is you move the beads, collect the beads, move from the different wash solution and the uduition.
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So again, we don't want to limit what do you do on the bench.
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This one is we do collaborate this small print form you can do anything you want.
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Same thing like a beads clean up.
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But we do have OEM solution especially for the Pike bio when you want to sharing DNA and this micro lab prep, we offer the sharing solution.
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So to shearing the DNA to the 15K to 22K base pair.
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So for your downstream reader, the otherwise you may have to use the Kowaros, the G tube and the each time you can only deal 1 sample.
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If you have a 96 sample, you can think or take a how long and how much resource.
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And then we of course, we also have the MP that we call the MP square based for the solid phase extraction.
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So you can do the philtre plate or you can just do the Kaijin spin tube with adapter.
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So we have a solution too.
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Now just quickly show with a nucleic acid.
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I see the case.
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We collaborate many different kinds of provider.
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And then now we talk about the solution for the NGS library prep.
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We have the smaller platform is also we have the IVDR in European side.
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It's CE proved for a lot of clinical lab uses.
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It's for the quick turn around and the important sample and the small throughput need this kind of process up to 24 samples.
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Then we have the bigger throughput system is called NGS STAR MOA.
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MOA means MPH96 and ODTC add-on.
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And so we call NGS MOA.
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Also we just recently last year we released the device we code is a FluroEye.
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So it's very compact and the only take one SBI space on the deck.
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So you can just do the quant on the deck.
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So you don't need to take it off deck to the cubic.
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So use this pick up by channel.
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Then it's go over the 96.
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Well, they we can give you the quant data.
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So with NGS we collaborate with many different NGS kit providers.
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So I just list a few which we work with the most here is just like overview of the Hamilton solution for the genomic research.
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So you can see and every single step we have a solution layer for you.
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So if you want to know the detail, you can go our website or come to the Booth 6 talk to us, please.
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So what make a Hamilton stand out from other liquid handler company is our pipetting excellence.
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So first I want to say we have a patent pipetting design.
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You may hear about the core technology is a compressed or in expansion.
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So we pick up a tip is not like a force on you know the tip.
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This way we generate the locker key airtight seal.
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So it's essential to guarantee you a low volume pipetting precision C and also we pick up perfectly 90° when you do the 96 piece clean up.
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It's very important that every single tips go to the centre of the whale to able to remove the residue of the ethanol.
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So the residue ethanol is very important, you know remove it important for your downstream yield and your laboratory quality.
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And of course the we have the automatic, the liquid detection, we have the contactable liquid detection function, we also have the pressure detector.
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So a lot of customer if you use the older deeper thing, you may deal with a harsh chemistry.
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So we able to do the liquid, the liquid extraction, we able to detect organic layer or increase layer by our pressure or the contactable liquid level detection.
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And of course we have the traceability, you know, barcode scan and auto load then we can especially human chemistry each single reagent, they have a barcode.
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So if you want to prevent the human error to mislocated the reagent, then we can read the barcode that dynamically we can assign, we know where your reagent loaded.
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So with all those feature is really ensure you have the, you know, reproducibility secure and the efficient the workflow.
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So This is why all those features make a Hamilton stand out.
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OK, so I'm now going to read a word by word about this challenge.
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I'm sure you're all expert than the than me.
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So I just say for the scRNA & spatial analysis, we have all technical challenges, we have biological challenges and a scale up challenges and Hamilton has the solution and also we collaborate with industrial leader like 10XGeonimcs, BD and the Parse and Bruker.
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We all have a partnership.
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We have a solution for all those, the single COO spatial biology solution.
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So I just want to quickly show is the whole transcriptome analysis, the workflow.
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So every single the blue bar is the automated solution and the quant we coloured green.
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So most people do offline, but with our device FluroEye, you can really do online to to counter the step too.
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So we also collaborate with Olink and we published a paper.
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You can read those paper to the proteomics solution and then I want to say we want to provide a solution and the support and the service is really important component for our principal.
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So we have over 80 service engineer over almost 80 applications specialist to you know just US territory.
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We have a equal amount in the Europe side to support our customer and really is a support customer satisfaction is our principle.
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We care a lot.
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So that's it.
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Thank you very much.