This presentation was delivered by Mikhail Okun, a field application scientist from ThermoFisher, who introduced the EVOS S1000 spatial imaging platform and discussed its impact on spatial biology. Okun began by emphasising the growing importance of spatial biology in modern research, noting its recognition as a transformative method for uncovering cellular patterns and aiding drug development. This context set the stage for ThermoFisher’s commitment to advancing the field through innovative imaging solutions.
Okun outlined the main approaches to fluorescence microscopy: conventional microscopy, cyclic imaging, and spectral imaging. Conventional microscopy is praised for its speed and ease of use but is limited by the number of markers it can detect. Cyclic imaging allows for a higher number of targets but can be time-consuming and may risk tissue damage. Spectral imaging, which is central to the EVOS S1000, enables the simultaneous detection of multiple labels in a single run, offering greater speed and tissue preservation. However, it introduces challenges such as dye interference, which are addressed through a process called unmixing.
Unmixing is essential for resolving overlapping fluorescent signals. The EVOS S1000 system provides a robust and transparent unmixing process, generating comprehensive reports that allow users to assess the quality of their results and troubleshoot as needed. The system supports a wide range of dyes, including Alexa Fluor and Opal dyes, and offers flexibility in assay design.
Okun highlighted the platform’s user-friendly design, aiming to make advanced microscopy accessible to a broad range of scientists. The software interface is intuitive, with straightforward workflows for image acquisition and analysis. The system supports both primary antibody conjugates for rapid labelling and tyramide signal amplification reagents for enhanced sensitivity, catering to diverse experimental needs.
The presentation concluded with examples of high-resolution imaging of cancer samples and the integration of RNA and protein targets. Okun stressed the EVOS S1000’s flexibility, speed, and reliability, positioning it as a tool that simplifies spatial biology and accelerates research progress. Ongoing development of protocols and support resources continues to enhance its utility for the scientific community.
